It took some ten years for this wound to cause his health to deteriorate to the point where it was life threatening and in August 1546 his youthful vigour was noticeable deserting him. As his health became a concern, his stools and sputum were regularly examined. Furthermore, the doctors at that time believed that the letting of his blood, in accordance with the waxing and waning of the moon could save him. Between August and December, the privy chamber spent increased amounts on his medical treatment to no avail.
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